Selecting the correct options in a kinase assay is critical in any type of testing situation. Often, challenges occur when the correct biochemical kinase assay is not selected, resulting in inaccuracies in results or inefficiencies in testing.
Companies such as BellBrook Labs work with pharmaceutical companies to develop custom kinase assays with specific targets. This is ideal for both high throughput screening requirements as well as for targeted testing for lead compounds. These mix-and-read format assays provide a clear indicator through the change in fluorescent properties through the displacement of the tracer during the kinase reaction.
The Importance of High Throughput Screening
The critical factor in choosing a biochemical kinase assay is the ability to have flexibility, reliability, and accuracy in the analysis. With high throughput screening, there is a greater testing capacity coupled with a shorter time in this phase of the drug development process. The HTS assay is also a cost-effective option both during analysis as well as in the overall drug concept to the market timeline.
Understanding the Parameters
Most of the kinase and ATPase assay options on the market are designed for small molecule inhibitors. They are also able to determine any enzymatic activity with the reagent, proving the mechanism of action studies as well as inhibitor selectivity profile development.
There are several assays designed to provide extremely high-quality data and reliability in testing using reagents capable of providing results within an enzyme family. These reagents should also be considered based on their stability in typical testing environments and applications, as this can have significant results on data from the assay.
Each type of assay will also have limitations for use as well as advantages in specific types of testing applications. Understanding the parameters for use in a given situation is always an essential factor in the selection of any biochemical assay.
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